Hypersensitive enhanced chemiluminescence (ECL) kit
The hypersensitive enhanced chemiluminescence reagent developed by HAKATA is used to detect the target band of the HRP (horseradish peroxidase) marker, and the sensitivity of the detection is up to the femtogram(fg) level. hECL optimizes the composition of the substrate and uses a new type of enhancer and oxidant. It has higher luminescence intensity, better stability, no toxic reagents and is safe.
High sensitivity: the highest level is 10-15 g.
Safety: reagent does not contain toxic chemicals that affect human health
Long lasting signal: exposure time of up to 30 minutes
High stability: store at 4˚C for more than two years
Economical: cost is less than similar reagents, and requires fewer antibodies
1. Diluting solution: mix hECL Luminol/Enhancer Reagent (A) and hECL Stabilized Peroxide Reagent (B) at a volume ratio of 1:1. Note: in order to get the best results, prepare the dilution immediately before use. When extracting the different components of hECL, use different pipettes to avoid reagent contamination.
2. Remove the membrane from the tray containing TBST or PBST buffer, carefully remove the excess buffer from the membrane, but do not dry the membrane.
3. Place the membrane, protein side up, and cover it with a good amount of working dilution. The standard amount needed to cover the membrane is 1 ml of working fluid, which can cover approximately 10 cm2 of membrane.
4. Incubate the membrane with working fluid for 1-5 minutes and ensure that the entire membrane surface is covered.
5. Acquire signals by autoradiographic film or imaging device. For an unknown signal, different exposure times may be needed to get the best results.
Product shelf life is two years if kept at 4˚C.
Note: for laboratory research use only