HAKATA Cell Freezing Medium
Cell freezing medium is a serum-free cell freezing medium, which is commonly used in various mammalian cell lines (tumor cells and conventional cells). Cryopreserved cells can be stored at -80˚C for a long period of time (>5 years). The formula of cell freezing medium is clear. It does not contain animal source proteins, and it does not contain serum. It can reduce contamination by all kinds of bacteria, viruses and mycoplasma to ensure the safety of frozen cells. The cryopreservation solution contains DMSO, glucose and other cellular nutrients, which can improve cell viability, and is suitable for serum-free culture cells.
The cell cryopreservation solution can be used directly.
Cells are cryopreserved directly in the refrigerator at -80˚C for a long period of time (> 5 years), and programmed cooling is not required.
It is sterile, with low likelihood of contamination by viruses, pathogens and mycoplasma.
The survival rate and viability of cells are high.
1. Using conventional methods, collect adherent or suspension cells at the logarithmic phase into a centrifuge tube.
2. Add the required number of suspended cells in a centrifuge tube, centrifuge at 1000 rpm for 5 minutes, and collect the pellet in the centrifuge tube.
3. Aliquot the cell mixture into labeled cryopreservation tubes. It is recommended that each tube contains 1 ml or 1.5 ml.
4. Directly place the cryopreservation tube into a -80˚C cryogenic refrigerator. The tubes can be frozen for a long period of time.
5. For long-term storage in liquid nitrogen, the cryopreservation tubes must be stored in a -80˚C refrigerator for at least one day before they can be moved to a liquid nitrogen tank.
Cryopreserved cell resuscitation steps
1．Remove frozen cells from the fridge and immediately place them in a 37˚C water bath to thaw. After the mixture of cells in the cryopreservation tube has completely melted, transfer it into a centrifuge tube containing the proper amount of fresh cell culture medium, centrifuge at 1000 rpm for 5 minutes, and collect the pellet in the centrifuge tube. Add fresh cell culture medium to the pellet and mix slowly and gently, and move the cell mixture into a cell culture dish.
2．After examination under the microscope, cells can be routinely cultured according to their needs and methods.
Transport at room temperature. Store at 4˚C for long periods of time. Store at -20˚C for 36 months.
Cell freezing medium is strictly monitored for endotoxin, osmotic pressure, pathogens, and pH to ensure that the product is free of bacteria, viruses and mycoplasma. For routine cell cryopreservation, cells can be stored at -80˚C for a long time (>5 years) with a cell survival rate of 90-98%.
1. After cryopreservation, cells should be moved to a refrigerator at -80˚C as soon as possible.
2. Cell freezing medium contains 10% DMSO; some cells are sensitive to DMSO. It is suggested that cells are cryopreserved in this product for at least 1 week to confirm the performance of the cryopreservation. Disclaimer: the company will not be responsible for any accidents caused by improper product use.